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low-glucose minimum essential medium (mem) containing neaa  (Procell Inc)

 
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    Procell Inc low-glucose minimum essential medium (mem) containing neaa
    Low Glucose Minimum Essential Medium (Mem) Containing Neaa, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/low-glucose minimum essential medium (mem) containing neaa/product/Procell Inc
    Average 90 stars, based on 1 article reviews
    low-glucose minimum essential medium (mem) containing neaa - by Bioz Stars, 2026-02
    90/100 stars

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    low glucose minimum essential medium mem  (Thermo Fisher)
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    Thermo Fisher low glucose minimum essential medium mem
    ( A ) 96-well plate reader measurement of the increase of EGFP fluorescence relative to Sirius in COS-7 cells transfected with the bicistronic Sirius-M 2 i3(417n)-EGFP plasmid and starved for 3 h respectively <t>in</t> <t>PBS</t> (left panel) ( n = 3 transfections, 16 wells) and HBSS (right panel) ( n = 1 transfection, 16 wells). Significance values reported in the graphs were determined by a one-tailed Student t test, p -values: **** p < 0.0001; ** 0.001< p <0.01. ( B ) Single-cell measurement of EGFP vs. SIRIUS expression in HEK293 cells, in basal conditions and upon starvation. Lines are linear fits (note log-log scale). ( C ) HEK293 cells were sequentially transfected with pMito-GFP1-10 (24 h) and M 2 -GFP11 (8 h, of which 6 h under starvation conditions) and then grown in full culture <t>medium</t> or ( D ) starved for 6 h after transfection of M 2 -GFP11. Shown are EGFP fluorescence (left, with DIC inset), Mitotracker (center), and overlay (right). Scale bars are 10 μm. Source data for panels A and B, and raw images related to panel C and D can be found in . IRES, internal ribosome entry site; PBS, phosphate-buffered saline.
    Low Glucose Minimum Essential Medium Mem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/low glucose minimum essential medium mem/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
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    low-glucose minimum essential medium (mem) containing neaa  (Procell Inc)
    90
    Procell Inc low-glucose minimum essential medium (mem) containing neaa
    ( A ) 96-well plate reader measurement of the increase of EGFP fluorescence relative to Sirius in COS-7 cells transfected with the bicistronic Sirius-M 2 i3(417n)-EGFP plasmid and starved for 3 h respectively <t>in</t> <t>PBS</t> (left panel) ( n = 3 transfections, 16 wells) and HBSS (right panel) ( n = 1 transfection, 16 wells). Significance values reported in the graphs were determined by a one-tailed Student t test, p -values: **** p < 0.0001; ** 0.001< p <0.01. ( B ) Single-cell measurement of EGFP vs. SIRIUS expression in HEK293 cells, in basal conditions and upon starvation. Lines are linear fits (note log-log scale). ( C ) HEK293 cells were sequentially transfected with pMito-GFP1-10 (24 h) and M 2 -GFP11 (8 h, of which 6 h under starvation conditions) and then grown in full culture <t>medium</t> or ( D ) starved for 6 h after transfection of M 2 -GFP11. Shown are EGFP fluorescence (left, with DIC inset), Mitotracker (center), and overlay (right). Scale bars are 10 μm. Source data for panels A and B, and raw images related to panel C and D can be found in . IRES, internal ribosome entry site; PBS, phosphate-buffered saline.
    Low Glucose Minimum Essential Medium (Mem) Containing Neaa, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/low-glucose minimum essential medium (mem) containing neaa/product/Procell Inc
    Average 90 stars, based on 1 article reviews
    low-glucose minimum essential medium (mem) containing neaa - by Bioz Stars, 2026-02
    90/100 stars
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    low-glucose minimum essential medium (mem)  (Procell Inc)
    90
    Procell Inc low-glucose minimum essential medium (mem)
    ( A ) 96-well plate reader measurement of the increase of EGFP fluorescence relative to Sirius in COS-7 cells transfected with the bicistronic Sirius-M 2 i3(417n)-EGFP plasmid and starved for 3 h respectively <t>in</t> <t>PBS</t> (left panel) ( n = 3 transfections, 16 wells) and HBSS (right panel) ( n = 1 transfection, 16 wells). Significance values reported in the graphs were determined by a one-tailed Student t test, p -values: **** p < 0.0001; ** 0.001< p <0.01. ( B ) Single-cell measurement of EGFP vs. SIRIUS expression in HEK293 cells, in basal conditions and upon starvation. Lines are linear fits (note log-log scale). ( C ) HEK293 cells were sequentially transfected with pMito-GFP1-10 (24 h) and M 2 -GFP11 (8 h, of which 6 h under starvation conditions) and then grown in full culture <t>medium</t> or ( D ) starved for 6 h after transfection of M 2 -GFP11. Shown are EGFP fluorescence (left, with DIC inset), Mitotracker (center), and overlay (right). Scale bars are 10 μm. Source data for panels A and B, and raw images related to panel C and D can be found in . IRES, internal ribosome entry site; PBS, phosphate-buffered saline.
    Low Glucose Minimum Essential Medium (Mem), supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/low-glucose minimum essential medium (mem)/product/Procell Inc
    Average 90 stars, based on 1 article reviews
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    low glucose minimum essential medium (mem)  (Corning Life Sciences)
    90
    Corning Life Sciences low glucose minimum essential medium (mem)
    ( A ) 96-well plate reader measurement of the increase of EGFP fluorescence relative to Sirius in COS-7 cells transfected with the bicistronic Sirius-M 2 i3(417n)-EGFP plasmid and starved for 3 h respectively <t>in</t> <t>PBS</t> (left panel) ( n = 3 transfections, 16 wells) and HBSS (right panel) ( n = 1 transfection, 16 wells). Significance values reported in the graphs were determined by a one-tailed Student t test, p -values: **** p < 0.0001; ** 0.001< p <0.01. ( B ) Single-cell measurement of EGFP vs. SIRIUS expression in HEK293 cells, in basal conditions and upon starvation. Lines are linear fits (note log-log scale). ( C ) HEK293 cells were sequentially transfected with pMito-GFP1-10 (24 h) and M 2 -GFP11 (8 h, of which 6 h under starvation conditions) and then grown in full culture <t>medium</t> or ( D ) starved for 6 h after transfection of M 2 -GFP11. Shown are EGFP fluorescence (left, with DIC inset), Mitotracker (center), and overlay (right). Scale bars are 10 μm. Source data for panels A and B, and raw images related to panel C and D can be found in . IRES, internal ribosome entry site; PBS, phosphate-buffered saline.
    Low Glucose Minimum Essential Medium (Mem), supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/low glucose minimum essential medium (mem)/product/Corning Life Sciences
    Average 90 stars, based on 1 article reviews
    low glucose minimum essential medium (mem) - by Bioz Stars, 2026-02
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    minimum essential medium (mem) with phenol red and low glucose  (Thermo Fisher)
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    Thermo Fisher minimum essential medium (mem) with phenol red and low glucose
    ( A ) 96-well plate reader measurement of the increase of EGFP fluorescence relative to Sirius in COS-7 cells transfected with the bicistronic Sirius-M 2 i3(417n)-EGFP plasmid and starved for 3 h respectively <t>in</t> <t>PBS</t> (left panel) ( n = 3 transfections, 16 wells) and HBSS (right panel) ( n = 1 transfection, 16 wells). Significance values reported in the graphs were determined by a one-tailed Student t test, p -values: **** p < 0.0001; ** 0.001< p <0.01. ( B ) Single-cell measurement of EGFP vs. SIRIUS expression in HEK293 cells, in basal conditions and upon starvation. Lines are linear fits (note log-log scale). ( C ) HEK293 cells were sequentially transfected with pMito-GFP1-10 (24 h) and M 2 -GFP11 (8 h, of which 6 h under starvation conditions) and then grown in full culture <t>medium</t> or ( D ) starved for 6 h after transfection of M 2 -GFP11. Shown are EGFP fluorescence (left, with DIC inset), Mitotracker (center), and overlay (right). Scale bars are 10 μm. Source data for panels A and B, and raw images related to panel C and D can be found in . IRES, internal ribosome entry site; PBS, phosphate-buffered saline.
    Minimum Essential Medium (Mem) With Phenol Red And Low Glucose, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/minimum essential medium (mem) with phenol red and low glucose/product/Thermo Fisher
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    low-glucose minimum essential medium (mem)  (HiMedia Laboratories)
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    HiMedia Laboratories low-glucose minimum essential medium (mem)
    ( A ) 96-well plate reader measurement of the increase of EGFP fluorescence relative to Sirius in COS-7 cells transfected with the bicistronic Sirius-M 2 i3(417n)-EGFP plasmid and starved for 3 h respectively <t>in</t> <t>PBS</t> (left panel) ( n = 3 transfections, 16 wells) and HBSS (right panel) ( n = 1 transfection, 16 wells). Significance values reported in the graphs were determined by a one-tailed Student t test, p -values: **** p < 0.0001; ** 0.001< p <0.01. ( B ) Single-cell measurement of EGFP vs. SIRIUS expression in HEK293 cells, in basal conditions and upon starvation. Lines are linear fits (note log-log scale). ( C ) HEK293 cells were sequentially transfected with pMito-GFP1-10 (24 h) and M 2 -GFP11 (8 h, of which 6 h under starvation conditions) and then grown in full culture <t>medium</t> or ( D ) starved for 6 h after transfection of M 2 -GFP11. Shown are EGFP fluorescence (left, with DIC inset), Mitotracker (center), and overlay (right). Scale bars are 10 μm. Source data for panels A and B, and raw images related to panel C and D can be found in . IRES, internal ribosome entry site; PBS, phosphate-buffered saline.
    Low Glucose Minimum Essential Medium (Mem), supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/low-glucose minimum essential medium (mem)/product/HiMedia Laboratories
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    minimum essential medium eagle (mem) with low glucose (1000 mg/l)  (Millipore)
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    Millipore minimum essential medium eagle (mem) with low glucose (1000 mg/l)
    ( A ) 96-well plate reader measurement of the increase of EGFP fluorescence relative to Sirius in COS-7 cells transfected with the bicistronic Sirius-M 2 i3(417n)-EGFP plasmid and starved for 3 h respectively <t>in</t> <t>PBS</t> (left panel) ( n = 3 transfections, 16 wells) and HBSS (right panel) ( n = 1 transfection, 16 wells). Significance values reported in the graphs were determined by a one-tailed Student t test, p -values: **** p < 0.0001; ** 0.001< p <0.01. ( B ) Single-cell measurement of EGFP vs. SIRIUS expression in HEK293 cells, in basal conditions and upon starvation. Lines are linear fits (note log-log scale). ( C ) HEK293 cells were sequentially transfected with pMito-GFP1-10 (24 h) and M 2 -GFP11 (8 h, of which 6 h under starvation conditions) and then grown in full culture <t>medium</t> or ( D ) starved for 6 h after transfection of M 2 -GFP11. Shown are EGFP fluorescence (left, with DIC inset), Mitotracker (center), and overlay (right). Scale bars are 10 μm. Source data for panels A and B, and raw images related to panel C and D can be found in . IRES, internal ribosome entry site; PBS, phosphate-buffered saline.
    Minimum Essential Medium Eagle (Mem) With Low Glucose (1000 Mg/L), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phenol red free low glucose minimum essential medium mem  (Thermo Fisher)
    99
    Thermo Fisher phenol red free low glucose minimum essential medium mem
    ( A ) 96-well plate reader measurement of the increase of EGFP fluorescence relative to Sirius in COS-7 cells transfected with the bicistronic Sirius-M 2 i3(417n)-EGFP plasmid and starved for 3 h respectively <t>in</t> <t>PBS</t> (left panel) ( n = 3 transfections, 16 wells) and HBSS (right panel) ( n = 1 transfection, 16 wells). Significance values reported in the graphs were determined by a one-tailed Student t test, p -values: **** p < 0.0001; ** 0.001< p <0.01. ( B ) Single-cell measurement of EGFP vs. SIRIUS expression in HEK293 cells, in basal conditions and upon starvation. Lines are linear fits (note log-log scale). ( C ) HEK293 cells were sequentially transfected with pMito-GFP1-10 (24 h) and M 2 -GFP11 (8 h, of which 6 h under starvation conditions) and then grown in full culture <t>medium</t> or ( D ) starved for 6 h after transfection of M 2 -GFP11. Shown are EGFP fluorescence (left, with DIC inset), Mitotracker (center), and overlay (right). Scale bars are 10 μm. Source data for panels A and B, and raw images related to panel C and D can be found in . IRES, internal ribosome entry site; PBS, phosphate-buffered saline.
    Phenol Red Free Low Glucose Minimum Essential Medium Mem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenol red free low glucose minimum essential medium mem/product/Thermo Fisher
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    dulbecco s minimum essential low glucose medium dmem  (Thermo Fisher)
    99
    Thermo Fisher dulbecco s minimum essential low glucose medium dmem
    ( A ) 96-well plate reader measurement of the increase of EGFP fluorescence relative to Sirius in COS-7 cells transfected with the bicistronic Sirius-M 2 i3(417n)-EGFP plasmid and starved for 3 h respectively <t>in</t> <t>PBS</t> (left panel) ( n = 3 transfections, 16 wells) and HBSS (right panel) ( n = 1 transfection, 16 wells). Significance values reported in the graphs were determined by a one-tailed Student t test, p -values: **** p < 0.0001; ** 0.001< p <0.01. ( B ) Single-cell measurement of EGFP vs. SIRIUS expression in HEK293 cells, in basal conditions and upon starvation. Lines are linear fits (note log-log scale). ( C ) HEK293 cells were sequentially transfected with pMito-GFP1-10 (24 h) and M 2 -GFP11 (8 h, of which 6 h under starvation conditions) and then grown in full culture <t>medium</t> or ( D ) starved for 6 h after transfection of M 2 -GFP11. Shown are EGFP fluorescence (left, with DIC inset), Mitotracker (center), and overlay (right). Scale bars are 10 μm. Source data for panels A and B, and raw images related to panel C and D can be found in . IRES, internal ribosome entry site; PBS, phosphate-buffered saline.
    Dulbecco S Minimum Essential Low Glucose Medium Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ( A ) 96-well plate reader measurement of the increase of EGFP fluorescence relative to Sirius in COS-7 cells transfected with the bicistronic Sirius-M 2 i3(417n)-EGFP plasmid and starved for 3 h respectively in PBS (left panel) ( n = 3 transfections, 16 wells) and HBSS (right panel) ( n = 1 transfection, 16 wells). Significance values reported in the graphs were determined by a one-tailed Student t test, p -values: **** p < 0.0001; ** 0.001< p <0.01. ( B ) Single-cell measurement of EGFP vs. SIRIUS expression in HEK293 cells, in basal conditions and upon starvation. Lines are linear fits (note log-log scale). ( C ) HEK293 cells were sequentially transfected with pMito-GFP1-10 (24 h) and M 2 -GFP11 (8 h, of which 6 h under starvation conditions) and then grown in full culture medium or ( D ) starved for 6 h after transfection of M 2 -GFP11. Shown are EGFP fluorescence (left, with DIC inset), Mitotracker (center), and overlay (right). Scale bars are 10 μm. Source data for panels A and B, and raw images related to panel C and D can be found in . IRES, internal ribosome entry site; PBS, phosphate-buffered saline.

    Journal: PLOS Biology

    Article Title: The C-terminus of the prototypical M2 muscarinic receptor localizes to the mitochondria and regulates cell respiration under stress conditions

    doi: 10.1371/journal.pbio.3002582

    Figure Lengend Snippet: ( A ) 96-well plate reader measurement of the increase of EGFP fluorescence relative to Sirius in COS-7 cells transfected with the bicistronic Sirius-M 2 i3(417n)-EGFP plasmid and starved for 3 h respectively in PBS (left panel) ( n = 3 transfections, 16 wells) and HBSS (right panel) ( n = 1 transfection, 16 wells). Significance values reported in the graphs were determined by a one-tailed Student t test, p -values: **** p < 0.0001; ** 0.001< p <0.01. ( B ) Single-cell measurement of EGFP vs. SIRIUS expression in HEK293 cells, in basal conditions and upon starvation. Lines are linear fits (note log-log scale). ( C ) HEK293 cells were sequentially transfected with pMito-GFP1-10 (24 h) and M 2 -GFP11 (8 h, of which 6 h under starvation conditions) and then grown in full culture medium or ( D ) starved for 6 h after transfection of M 2 -GFP11. Shown are EGFP fluorescence (left, with DIC inset), Mitotracker (center), and overlay (right). Scale bars are 10 μm. Source data for panels A and B, and raw images related to panel C and D can be found in . IRES, internal ribosome entry site; PBS, phosphate-buffered saline.

    Article Snippet: After washing each well with 2 ml PBS, the old medium was exchanged with 1.5 ml low glucose Minimum Essential Medium (MEM) (Gibco, Thermo Scientific) supplemented with 1% P/S, in order to create a stress-like cell starvation condition.

    Techniques: Fluorescence, Transfection, Plasmid Preparation, One-tailed Test, Expressing, Saline